Olive (Olea europaea L.) belongs to the Oleaceae family and it is mainly cultivated to produce olive oil, which is one of the cornerstones of the Mediterranean diet. The production of olive oil consists in several steps that produce several byproducts. In modern mill oil extraction processes (multiphase decanter) a by-product, called "paté", consisting in hydrated pulp with a high humidity (75-80%), is produced. After centrifugation of patè, the vegetative water (VW) can be recovered as supernatant. The VW, usually discarded as a toxic fraction for both natural beneficial and pathogenic microrganisms, is however a source rich of bioactive molecules such as phenols, secoiridoids and oligosaccharides effective in plant protection against pathogens. In the present research VW, derived from patè, have been fractionated by various stages of membrane filtration (MF) (i.e microfiltration, ultrafiltration and nanofiltration) in order to identify and quantify bioactive compounds useful in agriculture as phytopesticides and/or agents in crop protection against pathogens. The various MF fractions were analyzed by high-resolution NMR spectroscopy. Both the hydro and lyposoluble fractions indicated the presence of tyrosol, hydroxytirosol, oleuropein and lingstroside with a known antifungal and antimicrobial activity. Carbohydrate profile has been obtained by HPAE-PAD. Cell wall-derived pectin and oligogalacturonides (OGs) were identified. OGs are pectic fragments, elicitors of plant immune responses. Biological tests were performed to identify bioactive elicitors. Elicitation of cytosolic calcium transients, an early plant defense response to elicitors were observed in Arabidopsis plants expressing a GFP-based calcium biosensor (GCaMP3; gently provided by Alex Costa, University of Milan). In addition, a dose-dependent reduction of root growth in Arabidopsis seedlings, a well known physiological response induced by elicitors, was observed in specific MFT fractions. This finding highlights the potential of these by-products as biostimulant of plant defense responses. Financial support: ABASA project LazioInnova-Regione Lazio CUP: B81G18000770002
Glycomic and phytochemical profile of olive oil vegetative waters after membrane-based filtration to recover bioactive compounds / Sciubba, Fabio; Lionetti, Vincenzo; Pontiggia, Daniela; Pizzichini, Daniele; Salvati, Ascenzo; Mario Ticconi, Valerio; Miccheli, Alfredo; Bellincampi, Daniela. - (2021). (Intervento presentato al convegno Plant Biology Europe 2021 tenutosi a Tourin ( webinar meeting)).
Glycomic and phytochemical profile of olive oil vegetative waters after membrane-based filtration to recover bioactive compounds
Fabio Sciubba;Vincenzo Lionetti;Daniela Pontiggia
;Ascenzo Salvati;Alfredo Miccheli;Daniela Bellincampi
2021
Abstract
Olive (Olea europaea L.) belongs to the Oleaceae family and it is mainly cultivated to produce olive oil, which is one of the cornerstones of the Mediterranean diet. The production of olive oil consists in several steps that produce several byproducts. In modern mill oil extraction processes (multiphase decanter) a by-product, called "paté", consisting in hydrated pulp with a high humidity (75-80%), is produced. After centrifugation of patè, the vegetative water (VW) can be recovered as supernatant. The VW, usually discarded as a toxic fraction for both natural beneficial and pathogenic microrganisms, is however a source rich of bioactive molecules such as phenols, secoiridoids and oligosaccharides effective in plant protection against pathogens. In the present research VW, derived from patè, have been fractionated by various stages of membrane filtration (MF) (i.e microfiltration, ultrafiltration and nanofiltration) in order to identify and quantify bioactive compounds useful in agriculture as phytopesticides and/or agents in crop protection against pathogens. The various MF fractions were analyzed by high-resolution NMR spectroscopy. Both the hydro and lyposoluble fractions indicated the presence of tyrosol, hydroxytirosol, oleuropein and lingstroside with a known antifungal and antimicrobial activity. Carbohydrate profile has been obtained by HPAE-PAD. Cell wall-derived pectin and oligogalacturonides (OGs) were identified. OGs are pectic fragments, elicitors of plant immune responses. Biological tests were performed to identify bioactive elicitors. Elicitation of cytosolic calcium transients, an early plant defense response to elicitors were observed in Arabidopsis plants expressing a GFP-based calcium biosensor (GCaMP3; gently provided by Alex Costa, University of Milan). In addition, a dose-dependent reduction of root growth in Arabidopsis seedlings, a well known physiological response induced by elicitors, was observed in specific MFT fractions. This finding highlights the potential of these by-products as biostimulant of plant defense responses. Financial support: ABASA project LazioInnova-Regione Lazio CUP: B81G18000770002I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
